Journal: Scientific Reports

Article Title: Comparing and combining xevinapant with ATR and PARP inhibition for the radiosensitization of HPV-negative HNSCC cells

doi: 10.1038/s41598-026-38550-3

Figure Lengend Snippet: Cell death induction. Cells were seeded, after 3 h treated with substances and after an additional 2 h irradiated as indicated. 48 h later cells were harvested, stained for annexin V and DAPI and analyzed using flow cytometry. ( A ) Examples and gating. Events in the lower right rectangle were counted as early apoptosis and in the upper right rectangle as lytic cell death. Shown are HSC4 cells. ( B ) Quantification of early apoptosis and ( C ) quantification of lytic cell death. Significant differences to the respective irradiated or non-irradiated DMSO control are indicated with *, ** and *** indicating p < 0.05, p < 0.01 and p < 0.001, respectively (paired, two-tailed Student’s t-test). Asterisks in brackets indicate significant differences between irradiated and non-irradiated DMSO controls. Results are based on at least 4 individual experiments per cell line. Graphs display mean and standard deviation.

Article Snippet: HSC4 and SAS are also commercially available (AcceGen -#ABC-TC0420 & #ABL-TC0611, as of 05.01.2026).

Techniques: Irradiation, Staining, Flow Cytometry, Control, Two Tailed Test, Standard Deviation

Journal: Inflammopharmacology

Article Title: Selenium and selenoproteins: it’s role in regulation of inflammation

doi: 10.1007/s10787-020-00690-x

Figure Lengend Snippet: Selenium containing compounds for cancer treatment

Article Snippet: The compound produced noticeable anti-proliferative activities against three oral cancer cell lines (HSC-3, HSC-4, and SAS) at a concentration of 5–100 μM on treatment for 2–5 days (Endo et al. ).

Techniques: Concentration Assay, Produced, Expressing, Inhibition, Activity Assay, Activation Assay

Journal: Nature Communications

Article Title: A predominant enhancer co-amplified with the SOX2 oncogene is necessary and sufficient for its expression in squamous cancer

doi: 10.1038/s41467-021-27055-4

Figure Lengend Snippet: a BRD4 ChIP-seq profile at the SOX2 locus in KYSE140 cells with and without e1 repression. b Averaged BRD4 ChIP-seq profile, across BRD4 sites that harbor high-confidence SOX2 binding (*SOX2 ChIP-seq peaks containing SOX motifs) or the other BRD4 sites, in KYSE140 cells with and without e1 repression. c BRD4 ChIP-seq profile at the e1–e8 locus in LK2, NCI-H520, and HSC4 cells with and without e1 repression. d Top: PhastCons scores (0:1 range) representing the conservation level of DNA sequences in the e1 enhancer. Middle: distribution of JASPAR DNA motifs identified in the e1 enhancer. Bottom: CRISPR cutting sites that overlap with the identified DNA motifs. e RT-qPCR measuring expression changes of SOX2 in KYSE140 and LK2 cells after CRISPR-mediated disruption of each of the identified DNA motifs. The expression level was normalized to the sgAAVS1 control. n = 2 biologically independent experiments. *: combinatorial CRISPR cutting of SOX (2nd), AP1, RUNX, and STAT (2nd) motifs in KYSE140 cells, or SOX (2nd), AP1, SNAIL, and TCF motifs in LK2 cells. f ChIP-qPCR showing the relative enrichment of BRD4 at e1–e5 in KYSE140 and LK2 cells after combinatorial CRISPR cutting of the selected motifs. ChIP enrichment was normalized to DNA concentration of each sample (measured by Qubit) and then to sonicated genomic input. n = 2 biologically independent experiments. g Presence of the candidate functional motifs in the e1–e5 enhancers. Source data are provided as a Source Data file.

Article Snippet: Squamous cancer cell lines KYSE140, KYSE70, LK2, NCI-H520, HSC4, TE1, TE10, SKMES1, and RERFLCAI were obtained from the Broad Institute Cancer Cell Line Encyclopedia (CCLE) project , .

Techniques: ChIP-sequencing, Binding Assay, CRISPR, Quantitative RT-PCR, Expressing, Disruption, Control, ChIP-qPCR, Concentration Assay, Sonication, Functional Assay